The summary protein picture of the time-course hydrolysis of Hoki and Gemfish roe homogenate disclosed that all three proteases exhibited a non-selective hydrolytic fashion, indicating no evident specificity towards any from the roe proteins (Determine 6). Over-all, the final results indicated that proteins in equally Hoki and Gemfish roe homogenates were being hydrolyzed after some time by all 3 proteases and treatment plans. On the other hand, surprising bands with higher molecular body weight at 50–forty kDa ended up existing in FD-dL Hoki roe and Gemfish roe samples which was hydrolyzed by 10% FP-II.
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The two protein bands from 40 kDa to thirty kDa have been absent following 24 h incubation at 45 °C. For the remainder of the bands, various concentrations of all 3 proteases retained protein bands akin to their initial molar masses (Determine 6a,b). The presence of bands at very low molecular pounds in fish roe in advance of hydrolysis was unforeseen. Within the report by Ghelichi et al. [eight], slight peaks of a relatively reduced molecular bodyweight array (three–14 kDa) were being observed in defatted carp roe homogenate, most likely resulting from an autolytic process taking place in fish roe and/or maybe the defatting approach bringing about the development of low-molecular-pounds peptides.
Meskipun angka eight adalah angka hoki yang paling penting, kita tidak dapat mengesampingkan peran angka-angka lain, yang akan dijelaskan di bawah ini.
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